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From day 7 to day 10 the differences between control and AITC-treated seedlings were smaller than those observed during the second period, indicating that the effect of AITC on root growth persisted until day 10 but decreased over time (Supplementary Figure S2)

Hot water can irritate the injection site and potentially delay healing

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Throw it away if the vial has been at room temperature for more than a few hours