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After weighing, tissues were instantly placed in BIOPS buffer (calcium-EGTA (10 mM), free calcium (0.1 M), imidazole (20 mM), taurine (20 mM), morpholineethanesulfonic acid (50 mM), dithiothreitol (DTT, 0.5 mM), MgCl 2 (6.56 mM), ATP 5.77 (mM), phosphocreatine (15 mM)