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Before imaging, the growth medium was removed, then the cells were washed with PBS (2) and imaged in FluoroBrite DMEM

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Leppranta O, Myllrniemi M, Salmenkivi K, Kinnula VL, Keski-Oja J, Koli K: Reduced phosphorylation of the TGF-Beta signal transducer Smad2 in emphysematous human lung

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2013 Jul 11;4:189