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Corroborating these findings, immunoblot analysis of whole-kidney lysates demonstrated that UUO-induced upregulation of fibrotic (CCN2, FN1, COL1A1, and COL3A1) and ferroptotic (HMOX1) markers, alongside downregulation of GPX4, was more prominent in WT mice relative to KO littermates (Fig

PDL coating involved adding 100 g ml 1 PDL in sterile H 2 O for 30 min, followed by two washes with H 2 O and air-drying

[DOI] [Google Scholar] 138.Golden S.H., Selvin E., Cunningham K.E

Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NGF

doi: 10.1016/j.freeradbiomed.2021.02.046 125

When mitochondria are functioning normally, they generate minimal oxidative products and the bodys antioxidant defense systems keep them in check