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If this space cannot be adequately drained by the Eustachian tube, it can fill with fluid that eventually may become infected

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Cell culture Human primary T cells were cultured in X-Vivo 15 medium (Lonza) supplemented with 10% human heat-inactivated serum (Fisher), 10 ng ml 1 interleukin (IL)-7 (Miltenyi) and 10 ng ml 1 IL-15 (Miltenyi) at 37 C with 5% CO 2