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Situations or conditions that can cause vitamin B12 deficiency include: You are more likely to develop vitamin B12 deficiency if you have one or more of the following risk factors: Left untreated, vitamin B12 deficiency can cause lasting side effects that affect your nervous system and brain

Complementary DNA (cDNA) was made by reverse transcription of RNA (2 g) using Moloney murine leukemia virus reverse transcriptase (Promega, Madison, MI, USA) and labeled with Biotin-16-2-deoxy-uridine-5-triphosphate (Roche Diagnostics, Indianapolis, IN, USA)

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