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In brief, HUVECs were lysed in the Co-IP buffer containing 20 mM Tris-HCl (pH7.5), 100 mM NaCl, 0.5 mM EDTA, 0.5% NP-40, 0.5 mM PMSF, 0.5% protease inhibitor cocktail on ice for 20 min

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However, if visual field already severely damaged, this cellular preservation might not translate to functional improvement

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Angiogenesis induced by hepatocyte growth factor in non-infarcted myocardium and infarcted myocardium: up-regulation of essential transcription factor for angiogenesis, ets