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Data collection and integration were achieved using suitable software

The horseradish peroxidase conjugated secondary antibody (goat anti-mouse or goat anti-rabbit IgG), fluorescent isothiocyanate (FITC)-conjugated secondary antibody (goat anti-mouse IgG), 4,6-diamidino-2-phenylindole (DAPI) and Super Signal West Pico Trial Kit (enhanced chemiluminescence (ECL) chromogenic reagent kit) were purchased from Pierce, USA

The induction of protective cellular pathways, such as those involving vitagenes like sirtuins (a class of HDACs), has been proposed as a related neuroprotective strategy observed in other auditory pathologies like Menieres disease, which is characterized by tinnitus and considered a model of cochlear neurodegeneration (Scuto et al., 2020)

Long-term deficiency might lead to irreversible damage to the brain and nervous system

For such studies, bronchoconstriction, inflammation and lung function were primary endpoints