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Western blotting Cells were washed once with cold PBS and lysed in RIPA buffer (50 mM Tris-HCl pH 7.5, 125 mM NaCl, 1% NP-40, 0.25% Na-deoxycholate, 1mM Na-fluoride, 1 mM Na orthovanadate, phenylmethanesulfonylfluoride [PMSF], and 1% protease inhibitor cocktail), vortexed, and incubated on ice 20

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Uncontrolled sources may introduce unwanted variability and microbial contaminants that compromise the integrity of the reconstitution workflow

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Although AD and VaD share common vascular risk factors, the contribution of small artery disease to VaD pathogenesis may be greater than that of AD [120].In fact, studies have found that neuropathic changes can be evident 20 years before the onset of dementia and that there is a substantial neuronal loss in the MCI stage, which is considered a transitional state before the onset of dementia [121]