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2.4.1.8 9 U/mg soild 100 U/mg protein One unit is defined as the amount of enzyme that will produce 1.0 mole of D-Glucose from maltose per minute at pH 7.0 at 30C

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The coupling of each amino acid requires deprotection of the Fmoc group, which exposes the active site of the amino acid for coupling reaction with the next amino acid

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