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Techniques such as fluorescence recovery after photobleaching and dynamic contrast-enhanced magnetic resonance imaging (MRI) have demonstrated that interstitial fluid generally moves at a relatively slow velocity, ranging from 0.1 to 10 m/s, depending on the species under consideration [214]

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820,825,829 This suggests a conformational gating mechanism in HypB where GTP hydrolysis signals to the Ni II binding site through G3, thus initiating metal transfer to HypA, which itself later inserts Ni II into [NiFe]-hydrogenase (Figure 37, top )