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Bacillus subtilis
Rapid extraction of RT-PCR-ready RNA from cultured mammalian cells
5-mM solution of ATP and a 50-mM solution of MnCl2 are included
Recommended for membrane proteins with T7 vector
Storage Buffer: 50% glycerol containing 25 mM sodium acetate (pH 4
E. cloni-10G CLASSIC Electrocompetent Cells, 48 rxns (SixPacks) cell projects Bacillus subtilisTable 1. E. cloni Competent Cells for Cloning and Library Construction E. cloni Cell Lines Transformation Efficiency (cfu g pUC DNA) Cloning Methylated DNA BAC, Cosmid Cloning Blue White Screening 10G SUPREME Electrocompetent >4 1010 YES YES YES without IPTG induction 10G ELITE Electrocompetent >2 1010 YES NO YES without IPTG induction 10G CLASSIC Electrocompetent >5 109 YES NO YES without IPTG induction 10GF ELITE Electrocompetent >2 1010 YES NO YES
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