A specific role for CYP2B1 in pyridine oxidation of NNK was strongly suggested by its isolation and detection in rat liver, catalytic activity, and metabolism of NNK in human kidney cells transfected with the corresponding cDNA [23], induction in rat livers that were pretreated with phenobarbital [25], and decreased oxidative metabolism of NNK in rat liver, lung, and nasal microsomes that were treated with phenethyl isothiocyanate (PEITC), a chemical inhibitor of CYP2B1 [84]
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