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A comprehensive review of recent clinical data highlights that while folate deficiency is common among individuals with ASD, maternal folic acid supplementation does not correlate with higher risk of the disorder, indeed folate therapy has been associated with improved neurodevelopmental outcomes (Vasconcelos et al., 2025)

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In the forebrain, approximately 20% of synoptically released glutamate reaches the postsynaptic glutamate receptors from the synaptic cleft during synaptic transmission, and the remainder can reach the astrocytes for glutamate re-uptake [170]

Tissue collectionAt the time of scheduled termination, animals are sacrificed as follows

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Activated microglia decrease histone acetylation and Nrf2-inducible anti-oxidant defence in astrocytes: restoring effects of inhibitors of HDACs, p38 MAPK and GSK3