GCL belongs to the class of proteins that are sensitive to oxidative stress, and its expression is mainly under the regulation of the Nuclear factor (erythroid-derived 2)-like 2 (NFE2L2), a transcription factor that regulates a wide array of antioxidant responsive element-driven genes in various cell types ( the novo GSH biosynthesis is glutathione synthase (GS)
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TUNEL assay To test the neuronal destruction, the cell death levels of the DRG tissues were studied with the use of the in situ cell death detection kit (Roche Applied Science, PA, USA), according to the manufacturers instructions
Direct supplementation : One of the most obvious ways of boosting GSH levels is to supplement with it directly
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ALA has also been observed to enhance insulin-stimulated glucose uptake in skeletal muscle, thereby improving insulin sensitivity in patients with type 2 diabetes
