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Ann N Y Acad Sci 1340:3946 Buettner G, Schafer F (2006) Albert Szent-Gyrgyi: vitamin C identification

Briefly, tissue sections were incubated with primary antibodies at a suitable concentration at 4 C overnight

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TSC2 forms a heterodimeric complex with TSC1 that suppresses the mTOR complex 1 (mTORC1) activity by inhibiting the small GTPase Rheb, an essential mTORC1 activator [263]