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The cell lines were cultured in DMEM medium containing 4.5 g/ml glucose and L-glutamine (Gibco) supplemented with 10% heat-inactivated fetal calf serum (Gibco) and 100 U/ml penicillin and 100 g/ml streptomycin (Gibco)

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Direct muscle growth and strength Mechanisms: Increase protein synthesis in muscle cells Enhance amino acid uptake Stimulate satellite cell activation Promote muscle fiber hypertrophy Improve nutrient partitioning Peptides in this category: IGF-1 LR3 (most anabolic) Growth hormone secretagogues (CJC-1295, Ipamorelin) MGF (Mechano Growth Factor) Result: Bigger, stronger muscles that generate more force

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