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In the catalytic hydrolysis of glutamine, Ser286 at the active site of the GLS1 enzyme carries out a nucleophilic attack on the acyl carbon of glutamine, and the subsequent proton transfer of Tyr466 promotes the hydrolysis reaction

J Biol Chem 236:15341543 Turrens JF, Alexandre A, Lehninger AL (1985) Ubisemiquinone is the electron donor for superoxide formation by complex III of heart mitochondria

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