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The Ussing chamber technique is often utilised in animal studies where intestinal tissues may be obtained following sacrifice of the animals [41, 42] and in human studies of intestinal diseases where endoscopic biopsy samples may be obtained [43]

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RAAS inhibitors may reduce proteinuria-associated amino acid losses, indirectly affecting circulating BCAA pools, while SGLT2 inhibitors shift substrate utilization toward ketogenesis and fatty acid oxidation, potentially reducing BCAA oxidation

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