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However, a drawback of NP explants is that swelling pressure is no longer balanced by the AF and hyperosmolarity within the NP is difficult to maintain, which results in NP tissue swelling
After deparaffinization, it was kept in 3% H 2 O 2 for 10 min to inactivate endogenous peroxidase activity, and then it was washed in phosphate buffer solution (PBS)