intraperitoneally (i.p.) by injection or alternatively, topically at the burn, as a thin layer of cream (50 microg of BPC 157 dissolved in 2 ml of distilled water was mixed with 50 g of commercial neutral cream (also used as local vehicle-control)), while silver sulfadiazine 1% cream was a standard agent acting locally
Briefly, HaCaT keratinocytes were seeded at a density of 10,000 cells per well in 200 l fresh culture medium (DMEM supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin solution) into 96-well flat-bottomed microtitre plates (Costar, Corning, USA) and incubated at 37 C in humidified 5% CO 2 using AutoFlow NU-5510 Direct Heat CO 2 Incubator (NuAire, USA) for 24 hours before treatment
Kytidou K, Artola M, Overkleeft HS, Aerts JM
Guillemin GJ
Your condition is carefully examinednever guessed
[DOI] [PubMed] [Google Scholar] [16].Okamoto K, Toyokuni S, Uchida K, Ogawa O, Takenewa J, Kakehi Y, et al