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Extracts of the medium were incubated at 80 C for at least 15 min before being centrifuged at 15,000 g for 20 min

The treatment with Ru(II)-OH or NP2 showed a strong green fluorescence signal throughout the cell, indicative of the depletion of O 2 and therefore the O 2 consumption through the catalyst

This is the window that the Finkley 2005 trial captured, and the Carey/Pickart ultrasound work confirmed at the structural level: an average 28% increase in collagen density, with top responders at 51%